Wet Biobank

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(1) Blood sample collection

Blood collection is completed by the ward nurse.  The collected blood samples are centrally placed in the designated location of the 4°C refrigerator, and the Biobank staff transfer the samples to the sample bank at a fixed time every day in a low-temperature transfer box.  The samples arrive at the biobank and are immediately pre-processed to separate the plasma and blood cells. The whole process strictly follows standard operating procedures (SOPs).

(2) Tissue sample collection

Immediately after the tissue specimen was isolated, the staff of the biobank transferred it to the Department of pathology in a 4℃ low temperature transport box. After sampling under the guidance of the pathologist, the fresh tissue was immediately transferred to the liquid nitrogen tank for temporary storage, and the number and information were checked and stored in the warehouse in time.

(3) Urine sample collection

Urine and blood collection procedures are basically the same. Generally, morning urine collection is chosen. The urine was collected in sterile containers, and the collected urine was transferred by the biobank staff to the biobank with a low-temperature transfer box every day for pre-treatment. The urine supernatant and urine sediment were separated and stored in the biobank for storage.

(4) Sample processing flow

The corresponding basic patient information is exported from the hospital's HIS system, and the processing begins after checking and marking the information of each sample. The whole blood and urine samples are processed in the ultra-clean workbench, and the plasma, white membrane and blood cells are processed by the blood processing module of the automatic liquid workstation. The processed samples are temporarily stored in the water-cooled ultra-low temperature refrigerator at -80℃, and are regularly stored in the gas phase liquid nitrogen tank and the automatic gas phase liquid nitrogen storage system. After the tissue samples were transferred from the operating room to the Department of pathology, they were immediately sampled under the guidance of pathologists and temporarily stored in liquid nitrogen tanks. After the pathological diagnosis was confirmed, the tissue samples were transferred weekly to gas liquid nitrogen tanks for long-term storage.